Engineered resistance against proteinases.

Engineered resistance against proteinases.

Exogenous proteinase inhibitors are valuable and economically interesting protective biotechnological tools. We examined whether small proteinase inhibitors when fused to a selected target protein can protect the target from proteolytic degradation without simultaneously affecting the function and activity of the target domain. Two proteinase inhibitors were studied: a Kazal-type silk proteinas...

Engineered Soybean Cyst Nematode Resistance

Nematicidal effects of cysteine proteinases against sedentary plant parasitic nematodes.

Cysteine proteinases from the fruit and latex of plants, such as papaya, pineapple and fig, have previously been shown to have substantial anthelmintic efficacy, in vitro and in vivo, against a range of animal parasitic nematodes. In this paper, we describe the in vitro effects of these plant extracts against 2 sedentary plant parasitic nematodes of the genera Meloidogyne and Globodera. All the...

Bivalent DNA Vaccination with Genes Encoding Leishmania major Cysteine Proteinases Type I and II Protects Mice Against Infectious Challenge

A confederacy of proteinases.

The demolition of large buildings can be accomplished by a single explosive agent destroying a single class of structural components, namely weightbearing beams. Although the breakdown of tissues in disease has often been conceived to occur in an analogous manner, with a single proteinase degrading a single structural ECM protein, it has become clear that effective tissue destruction requires s...